Coding

Part:BBa_K5437018:Design

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


IspH converts HMBPP into DMAPP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Expressing E. coli. Codon optimization was still done with IDT Codon Optimization for best practice.


Source

Ecocyc provided FASTA sequence of gene.

References